Journal: BMC Infectious Diseases
Article Title: Rapid and accurate detection of RMP- and INH- resistant Mycobacterium tuberculosis in spinal tuberculosis specimens by CapitalBio™ DNA microarray: A prospective validation study
doi: 10.1186/1471-2334-12-303
Figure Lengend Snippet: Hybridization pattern of spinal tuberculosis specimens with the DNA microarray. a . Hybridization pattern of the M. tuberculosis strain identification (white frame) b . RMP resistance with the rpoB 531 (T C G->T T G) mutation, the hybridization signal of probe TCG531TTG (solid rectangle) being higher than corresponding wild-type probe (dashed rectangle) c . RMP resistance test: rpoB 516 (G A C->G T C) mutation, the hybridization signal of probe GAC516GTC (solid rectangle) being higher than corresponding wild-type probe (dashed rectangle) d , e . INH resistance test: katG 315 (A G C->A C C) mutation, the hybridisation signal of probe katG AGC315ACC (solid rectangles) were higher than corresponding wild-type probe (dashed rectangles) e. INH resistance test: inhA -15 C-T mutation, the hybridisation signal of probe inhA 15C→T (solid rectangles) were higher than corresponding wild-type probe (dashed rectangles).
Article Snippet: Specimens collected during surgery from the tuberculosis patients were subjected to M. tuberculosis species identification and drug-resistance detection by the CapitalBio™ DNA microarray, and results were compared with those obtained from the absolute concentration drug susceptibility testing.
Techniques: Hybridization, Microarray, Mutagenesis